A field experiment was carried out during the spring season of 2023 in two locations: the first one in Erbil city (Grdarasha field), and the second in the region of Khabat, located it become from west of Erbil province and (37) km far away from the city center, also the soil was silty clay loam texture in both locations, the aim of this study to know the effect of nano-boron on three variety of Sunflower on growth, yield and quality and identify the suitable variety for achieving the highest productivity, in addition to estimating their content of oils and fatty acids. The experiment was conducted using a split-plot randomized complete block design (RCBD) with three replicates and two factors, the first factor included four concentrations of nano-boron (0, 1, 2, 3 g/L), besides the second factor included three varieties of sunflower (Leviathan, Aqmar, Banam), Seeds were planted at both locations on March 29, 2023, and March 30, 2023 in Erbil and Khabat sites, at a depth of (1-3 cm) and a distance of 40 cm between plants  from rows. Nano-boron was sprayed on the plants once just before flowering. The results showed the following: It showed significant differences between nano-boron fertilizer and varieties in all studied traits. Nano-boron recorded the highest averages in some vegetative growth traits such as height, leaf area, chlorophyll content in plants, disc diameter, number of seeds per disc, 1000-seed weight, seed yield, oil percentage in seeds, oil yield, and fatty acids content in both locations. Additionally, the results also showed a clear significant superiority of the fertilizer levels (3g /L), which it gave the highest averages, the varieties recorded significant differences among them so Aqmar variety recorded the highest averages in most of the studied traits such as number of seeds per disc, seed yield, oil percentage, and oil yield in both experimental sites, while the Leviathan variety gave and recorded the highest means in some growth characteristics such as height and chlorophyll content in both sites, and fatty acids content in the Erbil place. Moreover, the Aqmar variety outperformed and showed a high content of fatty acids in the Khabat location, as for the interaction between nano-boron fertilizer and varieties, it had a significant impact on some of the studied traits, with the highest number of seeds per disc, seed yield, and oil yield obtained from the interaction between the dose (3g/L) of nano-boron fertilizer with Banam variety.